d(-)-Lactic Cytochrome c Reductase, a Flavoprotein from Yeast
نویسندگان
چکیده
منابع مشابه
Induction of D(-)- and L-lactic cytochrome c reductase in yeast.
Aerobically grown bakers’ yeast has been shown to contain two different lactic cytochrome c reductases, one of which is specific for L( +)-lactate (1) and the other of which is specific for D( -)-lactate (2). Neither of these two enzymes is present in anaerobically grown yeast, which contains instead a D( -)lactic dehydrogenase that does not reduce cytochrome c. No L( +)-lactic dehydrogenase is...
متن کاملThe kinetics of yeast D-lactic cytochrome c reductase studied by means of integrated velocity equations.
In a recent publication (l), the kinetics of n-lactic cytochrome tration of reaction products). The interpretation of the +-conc reductase was studied by measurements of initial velocities. &ants is given by Dalziel (3) for different reaction schemes; for The enzyme showed a kinetic behavior which appeared to agree the preceding reaction scheme we have: with the general equation suggested by Al...
متن کاملChapter 10 Purification of the cytochrome C reductase/cytochrome C oxidase super complex of yeast mitochondria.
The protein complexes of the respiratory chain interact by forming large protein particles called respiratory supercomplexes or "respirasomes". Biochemical characterization of these particles proved to be difficult because of their instability. Here we describe a strategy to isolate and characterize the cytochrome c reductase/cytochrome c oxidase supercomplex of yeast, also termed the III + IV ...
متن کاملA structural model of the cytochrome C reductase/oxidase supercomplex from yeast mitochondria.
Mitochondrial respiratory chain complexes are arranged in supercomplexes within the inner membrane. Interaction of cytochrome c reductase (complex III) and cytochrome c oxidase (complex IV) was investigated in Saccharomyces cerevisiae. Projection maps at 15 A resolution of supercomplexes III(2) + IV(1) and III(2) + IV(2) were obtained by electron microscopy. Based on a comparison of our maps wi...
متن کاملCytochrome c Oxidase from Bakers’ Yeast
Purified cytochrome c oxidase from bakers’ yeast can be resolved into six polypeptide bands by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The apparent molecular weights of these components are I, 42,000; II, 34,500; III, 23,000; IV, 14,000; V, 12,500; and VI, 9,500. (Although Component V actually consists of two distinct polypeptide species, it will be regarde...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1961
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)64331-5